Evaluation of Anti-cancer Activities of Cranberries Juice Concentrate in Osteosarcoma Cell Lines (MG-63)

Abstract: Aim/Background: Osteosarcoma is one of the prevalent cancers occurring mostly in adolescents and has a high risk malignancy. With complications involved current treatment strategies, alternates including use phytochemicals have gained fame. Cranberries are known for their exceptional health benefits been explored effective activities various cancers. The study aimed at evaluating anti-cancer properties cranberry juice concentrate (CJC) on MG-63 cell line human osteosarcoma, by investigating its apoptotic activity through changes viability mitochondrial membrane potential. Materials Methods: Cranberry was obtained pulverization lyophilization. cells were treated with 12.5-800 μg/mL CJC incubated 24, 48, 72 hr. percentage IC50 values obtained. potential nuclear examined. induction apoptosis studied flow cytometer using BD Quest 7.5.3 software. GraphPad Prism used statistical analysis significant p-value <0.05. Results: 847.9, 637.4, 440.6 hr respectively. Change morphology observed following incubation CJC. Flow cytometric shows detected early late apoptoic stages after Conclusion: Our result suggests that effects osteosarcoma can be considered to supplement conventional therapeutic strategies. Keywords: Apoptosis, Cranberry, MG-63, Osteosarcoma, Phytochemicals.